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Tryptic soy broth or Trypticase soy broth (frequently abbreviated as TSB) is used in microbiology laboratories as a culture broth to grow aerobic and facultative anaerobic bacteria. It is a general purpose medium that is routinely used to grow bacteria which tend to have high nutritional requirements (i.e., they are fastidious ).
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Colonies of Micrococcus luteus on Tryptic Soy Agar. Cultivation 48 hours, 37°C. Trypticase soy agar or Tryptic soy agar (TSA) is a growth media for the culturing of moderately to non fastidious bacteria. It is a general-purpose, non-selective media providing enough nutrients to allow for a wide variety of microorganisms to grow.
Lysogeny broth is used to culture Escherichia coli. [10] MacConkey agar is a selective and differential medium used to differentiate between gram-negative bacteria while inhibiting the growth of gram-positive bacteria. Adding bile salts and crystal violet to the agar inhibits the growth of most gram-positive bacteria, making MacConkey agar ...
YPD medium bottle and YPD agar plate. YEPD or yeast extract peptone dextrose, also often abbreviated as YPD, is a complete medium for yeast growth. It contains yeast extract, peptone, double-distilled water, and glucose (dextrose). [1]
Nutrient broth has the same composition, but lacks agar. [ 2 ] [ 3 ] These ingredients are combined and boiled for approximately one minute to ensure they are mixed and then sterilized by autoclaving, typically at 121 °C (250 °F) for 15 minutes.
Plate count agar (PCA), also called standard methods agar (SMA), is a microbiological growth medium commonly used to assess or to monitor "total" or viable bacterial growth of a sample.
YM Agar and Broth, is a selective growth medium with low pH useful for cultivating yeasts, molds, or other acid-tolerant or acidophilic organisms, while deterring growth of most bacteria and other acid intolerant organisms. [1] It is malt extract medium modified by the addition of yeast extract and peptone. [2]