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Tryptic soy broth or Trypticase soy broth (frequently abbreviated as TSB) is used in microbiology laboratories as a culture broth to grow aerobic and facultative anaerobic bacteria. It is a general purpose medium that is routinely used to grow bacteria which tend to have high nutritional requirements (i.e., they are fastidious ).
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
YM Agar and Broth, is a selective growth medium with low pH useful for cultivating yeasts, molds, or other acid-tolerant or acidophilic organisms, while deterring growth of most bacteria and other acid intolerant organisms. [1] It is malt extract medium modified by the addition of yeast extract and peptone. [2]
YPD medium bottle and YPD agar plate. YEPD or yeast extract peptone dextrose, also often abbreviated as YPD, is a complete medium for yeast growth. It contains yeast extract, peptone, double-distilled water, and glucose (dextrose). [1]
Colonies of Micrococcus luteus on Tryptic Soy Agar. Cultivation 48 hours, 37°C. Trypticase soy agar or Tryptic soy agar (TSA) is a growth media for the culturing of moderately to non fastidious bacteria. It is a general-purpose, non-selective media providing enough nutrients to allow for a wide variety of microorganisms to grow.
Lysogeny broth is used to culture Escherichia coli. [10] MacConkey agar is a selective and differential medium used to differentiate between gram-negative bacteria while inhibiting the growth of gram-positive bacteria. Adding bile salts and crystal violet to the agar inhibits the growth of most gram-positive bacteria, making MacConkey agar ...
The growth of Neisseria meningitidis colonies on New York City Medium Agar. Streak the specimen as soon as possible after it is received in the laboratory.If material is being cultured directly from a swab, proceed as follows: [10]
Nutrient broth has the same composition, but lacks agar. [ 2 ] [ 3 ] These ingredients are combined and boiled for approximately one minute to ensure they are mixed and then sterilized by autoclaving, typically at 121 °C (250 °F) for 15 minutes.