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Testicular sperm extraction (TESE) is a surgical procedure in which a small portion of tissue is removed from the testicle and any viable sperm cells from that tissue are extracted for use in further procedures, most commonly intracytoplasmic sperm injection (ICSI) as part of in vitro fertilisation (IVF). [1]
Percutaneous epididymal sperm aspiration (PESA) is a technique used to determine sperm counts in the event of a possible blockage of the vas deferens. It is an alternative to microepidydimal sperm aspiration (MESA), and aims to address the technical difficulty and cost of MESA. [ 1 ]
Surgical extraction, if for example a blockage in the vas deferens is suspected to impede fertility, semen can be taken directly from the epididymis. Such a collection is called percutaneous epididymal sperm aspiration (PESA). Alternatively, the testicular tissue itself, instead of the sperm produced can be investigated.
In a review of 1,890 patients who had sperm retrieval results compared with aspiration found that testicular sperm extraction (TESE) with multiple biopsies was 2-fold more effective at finding sperm and microTESE (a microsurgical search for sperm within the testis) was 1.5-fold more effective at finding sperm [25] From a review n=159 cases of ...
The extraction of sperm from epididymis is also known as percutaneous epididymal sperm aspiration (PESA) and extraction of sperm from testicle is also known as testicular sperm aspiration (TESA). Depending on the total amount of spermatozoa in the semen sample, either low or high, it can be just washed or capacitated via swim-up or gradients ...
Procedure to extract sperm for IVF include percutaneous epididymal sperm aspiration (PESA procedure), testicular sperm extraction (TESE procedure) and open testicular biopsy. Needle aspiration in a PESA procedure invariably causes trauma to the epididymal tubule and TESE procedures may damage the intra testicular collecting system (rete testis).
Methods of semen collection include masturbation, condom collection, and epididymal extraction. The sample should never be obtained through coitus interruptus as some portion of the ejaculate could be lost, bacterial contamination could occur, or the acidic vaginal pH could be detrimental for sperm motility.
Microscopic testicular sperm extraction is a microsurgical procedure that extracts sperm straight from the patient's testes. It has a substantially greater sperm retrieval success rate than testicular sperm aspiration, which is performed using a simple needle stick but does not require a microscopic surgeon. [1]