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Ligation (molecular biology) A sticky end ligation. Ligation is the joining of two nucleotides, or two nucleic acid fragments, into a single polymeric chain through the action of an enzyme known as a ligase. The reaction involves the formation of a phosphodiester bond between the 3'-hydroxyl terminus of one nucleotide and the 5'-phosphoryl ...
t. e. Molecular biology / məˈlɛkjʊlər / is a branch of biology that seeks to understand the molecular basis of biological activity in and between cells, including biomolecular synthesis, modification, mechanisms, and interactions. [1][2][3] Though cells and other microscopic structures had been observed in living organisms as early as the ...
Cell lineage. Cell lineage denotes the developmental history of a tissue or organ from the fertilized egg. [1] This is based on the tracking of an organism's cellular ancestry due to the cell divisions and relocation as time progresses, this starts with the originator cells and finishing with a mature cell that can no longer divide.
A biological network is a method of representing systems as complex sets of binary interactions or relations between various biological entities. [1] In general, networks or graphs are used to capture relationships between entities or objects. [1] A typical graphing representation consists of a set of nodes connected by edges.
Maxam–Gilbert sequencing. Maxam–Gilbert sequencing is a method of DNA sequencing developed by Allan Maxam and Walter Gilbert in 1976–1977. This method is based on nucleobase -specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides. [1]
Transduction is the process by which foreign DNA is introduced into a cell by a virus or viral vector. [1] An example is the viral transfer of DNA from one bacterium to another and hence an example of horizontal gene transfer. [2] Transduction does not require physical contact between the cell donating the DNA and the cell receiving the DNA ...
1. All living organisms are composed of one or more cells. 2. The cell is the most basic unit of life. Schleiden's theory of free cell formation through crystallization was refuted in the 1850s by Robert Remak, Rudolf Virchow, and Albert Kolliker. [5] In 1855, Rudolf Virchow added the third tenet to cell theory.
The initial development of the cell marked the passage from prebiotic chemistry to partitioned units resembling modern cells. The final transition to living entities that fulfill all the definitions of modern cells depended on the ability to evolve effectively by natural selection. This transition has been called the Darwinian transition.