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Sequence coverage (or depth) is the number of unique reads that include a given nucleotide in the reconstructed sequence. [1] [2] Deep sequencing refers to the general concept of aiming for high number of unique reads of each region of a sequence. [3] Physical coverage, the cumulative length of reads or read pairs expressed as a multiple of ...
These concepts are already being exploited in genetic engineering. [20] For example, a research team has actually constructed a PAC shuttle vector that creates a library representing two-fold coverage of the human genome. [17] This could serve as an incredible resource to identify genes, or sets of genes, causing disease.
DNA segment with three genes, showing a double recombination event. If the individual recombination rates (between A and B; and between B and C) are known, then the c.o.c. between the regions AB and BC can be calculated from the rate of double recombination.
Fisher's fundamental theorem of natural selection is an idea about genetic variance [1] [2] in population genetics developed by the statistician and evolutionary biologist Ronald Fisher. The proper way of applying the abstract mathematics of the theorem to actual biology has been a matter of some debate, however, it is a true theorem.
The coding region of a gene, also known as the coding DNA sequence (CDS), is the portion of a gene's DNA or RNA that codes for a protein. [1] Studying the length, composition, regulation, splicing, structures, and functions of coding regions compared to non-coding regions over different species and time periods can provide a significant amount of important information regarding gene ...
The proportion of segregating sites within a gene is an important statistic in population genetics since it can be used to estimate mutation rate assuming no selection. For example it is used to calculate the Tajima's D neutral evolution statistic. A sequence alignment, produced by ClustalO, of mammalian histone proteins.
Gene structure is the organisation of specialised sequence elements within a gene. Genes contain most of the information necessary for living cells to survive and reproduce. [ 1 ] [ 2 ] In most organisms, genes are made of DNA, where the particular DNA sequence determines the function of the gene.
This situation can be important if, for example, the genetic marker is linked to a disease locus with a strong selection against heterozygous individuals. This observation suggests to shift the statistical model from a set of independent transmissions to a set of independent children (see Sasieni (1997) [ 4 ] for the corresponding problem in ...
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