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The Adamkiewicz reaction is part of a biochemical test used to detect the presence of the amino acid tryptophan in proteins. When concentrated sulfuric acid is combined with a solution of protein and glyoxylic acid, a red/purple colour is produced. It was named after its discoverer, Albert Wojciech Adamkiewicz.
Example of saponification reaction of a triglyceride molecule (left) with potassium hydroxide (KOH) yielding glycerol (purple) and salts of fatty acids ().. Saponification value or saponification number (SV or SN) represents the number of milligrams of potassium hydroxide (KOH) or sodium hydroxide (NaOH) required to saponify one gram of fat under the conditions specified.
Salkowski test can also be used to test the presence of indoles (crystalline alkaloids that are a degradation products of proteins, containing tryptophan [10]). In such cases a sample is treated with nitric acid and 2% solution of potassium nitrite , with positive reaction being shown by presence of red colour.
Saponification is a process of cleaving esters into carboxylate salts and alcohols by the action of aqueous alkali. Typically aqueous sodium hydroxide solutions are used. [1] [2] It is an important type of alkaline hydrolysis. When the carboxylate is long chain, its salt is called a soap. The saponification of ethyl acetate gives sodium acetate ...
Alkaline hydrolysis of esters is also known as saponification. A base such as sodium hydroxide is required in stochiometric amounts. Unlike acid-catalyzed ester hydrolysis, it is not an equilibrium reaction and proceeds to completion.
The Reichert value is an indicator of how much volatile fatty acid can be extracted from a particular fat or oil through saponification. It is equal to the number of millilitres of 0.1 normal hydroxide solution necessary for the neutralization of the water-soluble volatile fatty acids distilled and filtered from 5 grams of a given saponified fat.
Inhalation of the Denigés' reagent can result in acute poisoning: causing tightness in the chest, difficulties breathing, coughing and pain. Exposure of mercury sulfate to the eyes can cause ulceration of conjunctiva and cornea. If mercury sulfate is exposed to the skin it may cause sensitization dermatitis.
It is an indicator of how much volatile fatty acid can be extracted from fat through saponification. It is equal to the number of milliliters of 0.1 normal alkali solution necessary for the neutralization of the water-insoluble volatile fatty acids distilled and filtered from 5 grams of a given saponified fat.