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[1] [2] Once stained as part of a sample, these organisms can resist the acid and/or ethanol-based decolorization procedures common in many staining protocols, hence the name acid-fast. [ 2 ] The mechanisms of acid-fastness vary by species although the most well-known example is in the genus Mycobacterium , which includes the species ...
The acid-fast staining method, in conjunction with auramine phenol staining, serves as the standard diagnostic tool and is widely accessible for rapidly diagnosing tuberculosis (caused by Mycobacterium tuberculosis) and other diseases caused by atypical mycobacteria, such as leprosy (caused by Mycobacterium leprae) and Mycobacterium avium ...
English: This is a diagram of the basic steps of a Ziehl-Neelsen (Acid Fast) staining procedure File:Basic steps of acid fast staining procedure.svg is a vector version of this file. It should be used in place of this PDF file when not inferior.
The Kinyoun method can be modified as a weak acid fast stain, which uses 0.5–1.0% sulfuric acid instead of hydrochloric acid.The weak acid fast stain, in addition to staining Mycobacteria, will also stain organisms that are not able to maintain the carbol fuchsin after decolorizing with HCl, such as Nocardia species and Cryptosporidium.
- Mycobacterium avium complex: Wet-appearing colonies - M. gordonae: Yellowish colonies. In the Runyon classification, both bacteria are nonchromogens. They can be differentiated from M. tuberculosis and each other by commercially available DNA probes. [4]: 245 They are characterized as Gram-positive, nonmotile, acid-fast, short to long rods.
Mycobacterium smegmatis is an acid-fast bacterial species in the phylum Actinomycetota and the genus Mycobacterium. It is 3.0 to 5.0 μm long with a bacillus shape and can be stained by Ziehl–Neelsen method and the auramine-rhodamine fluorescent method.
Mycobacterium phlei is a species of acid-fast bacteria in the genus Mycobacterium. [1] It is characterized as one of the fast-growing mycobacteria. M. phlei has only occasionally been isolated in human infections, and patients infected with M. phlei generally respond well to anti-mycobacterial therapy. M. phlei has an unusually high GC-content ...
M. Mycobacterium; Mycobacterium africanum; Mycobacterium asiaticum; Mycobacterium avium avium; Mycobacterium avium hominissuis; Mycobacterium avium silvaticum