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  2. File:CRISPR Sterics.pdf - Wikipedia

    en.wikipedia.org/wiki/File:CRISPR_Sterics.pdf

    You are free: to share – to copy, distribute and transmit the work; to remix – to adapt the work; Under the following conditions: attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made.

  3. CRISPR interference - Wikipedia

    en.wikipedia.org/wiki/CRISPR_interference

    CRISPR interference (CRISPRi) is a genetic perturbation technique that allows for sequence-specific repression of gene expression in prokaryotic and eukaryotic cells. [1] It was first developed by Stanley Qi and colleagues in the laboratories of Wendell Lim , Adam Arkin, Jonathan Weissman , and Jennifer Doudna . [ 2 ]

  4. File:CRISPRflowchart.pdf - Wikipedia

    en.wikipedia.org/wiki/File:CRISPRflowchart.pdf

    You are free: to share – to copy, distribute and transmit the work; to remix – to adapt the work; Under the following conditions: attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made.

  5. CRISPR gene editing - Wikipedia

    en.wikipedia.org/wiki/CRISPR_gene_editing

    Apart from knock-out there are also knock-down (CRISPRi) and activation (CRISPRa) libraries, which use the ability of proteolytically deactivated Cas9-fusion proteins (dCas9) to bind target DNA, which means that a gene of interest is not cut but is over-expressed or repressed. It made CRISPR/Cas9 system even more interesting in gene editing.

  6. CRISPR - Wikipedia

    en.wikipedia.org/wiki/CRISPR

    Part of a series on: Genetic engineering Genetically modified organisms; Bacteria; Viruses; Animals. Mammals; Fish; Insects; Plants. Maize/corn; Rice; Soybean; Potato ...

  7. Dying To Be Free - The Huffington Post

    projects.huffingtonpost.com/dying-to-be-free...

    The last image we have of Patrick Cagey is of his first moments as a free man. He has just walked out of a 30-day drug treatment center in Georgetown, Kentucky, dressed in gym clothes and carrying a Nike duffel bag. The moment reminds his father of Patrick’s graduation from college, and he takes a picture of his son with his cell phone.

  8. Genome-wide CRISPR-Cas9 knockout screens - Wikipedia

    en.wikipedia.org/wiki/Genome-wide_CRISPR-Cas9...

    The exact protocol for lentiviral production will vary depending on the research aim and applied library. [35] [43] [44] If a two vector-system is used, for example, cells are sequentially transduced with Cas9 and sgRNA in a two-step procedure. [35] [44] Although more complex, this has the advantage of a higher titre for the sgRNA library virus ...

  9. Perturb-seq - Wikipedia

    en.wikipedia.org/wiki/Perturb-seq

    CRISPR interference (CRISPRi) on the other hand utilizes a catalytically inactive nuclease to physically block RNA polymerase, effectively preventing or halting transcription. [8] Perturb-seq has been utilized with both the knockout and CRISPRi approaches in the Dixit et al. paper [2] and the Adamson et al. paper, [1] respectively.