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The ligase chain reaction (LCR) is a method of DNA amplification. The ligase chain reaction (LCR) is an amplification process that differs from polymerase chain reaction (PCR) in that it involves a thermostable ligase to join two probes or other molecules together which can then be amplified by standard PCR cycling. [ 1 ]
An amplicon sequence template that has been prepared for amplification. The target sequence to be amplified is colored green. In molecular biology, an amplicon is a piece of DNA or RNA that is the source and/or product of amplification or replication events.
The liquid junction potential cannot be measured directly but calculated. The electromotive force (EMF) of a concentration cell with transference includes the liquid junction potential. The EMF of a concentration cell without transport is: =
The laminar finite rate model computes the chemical source terms using the Arrhenius expressions and ignores turbulence fluctuations. This model provides with the exact solution for laminar flames but gives inaccurate solution for turbulent flames, in which turbulence highly affects the chemistry reaction rates, due to highly non-linear Arrhenius chemical kinetics.
Another example is the unimolecular nucleophilic substitution (S N 1) reaction in organic chemistry, where it is the first, rate-determining step that is unimolecular. A specific case is the basic hydrolysis of tert-butyl bromide (t-C 4 H 9 Br) by aqueous sodium hydroxide. The mechanism has two steps (where R denotes the tert-butyl radical t-C ...
When working with limiting amount of sample, an analyst might need to make a single addition, but it is generally considered a best practice to make at least two additions whenever possible. [5] Note that this is not limited to liquid samples. In atomic absorption spectroscopy, for example, standard additions are often used with solid as the ...
While the updated calculation has a lower impact than the original one, “this study just reveals one of the ways we’re exposed to flame retardants in our daily life,” Liu says. “We’re ...
In enzymology, a leucoanthocyanidin reductase (EC 1.17.1.3) (LAR, aka leucocyanidin reductase or LCR) is an enzyme that catalyzes the chemical reaction (2R,3S)-catechin + NADP + + H 2 O ⇌ {\displaystyle \rightleftharpoons } 2,3-trans-3,4-cis-leucocyanidin + NADPH + H +