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STR analysis is a tool in forensic analysis that evaluates specific STR regions found on nuclear DNA. The variable (polymorphic) nature of the STR regions that are analyzed for forensic testing intensifies the discrimination between one DNA profile and another. [3] Scientific tools such as FBI approved STRmix incorporate this research technique.
Developed in 1991, [10] DQ alpha testing was the first forensic DNA technique that utilized the polymerase chain reaction. [11] This technique allowed for the use of far fewer cells than RFLP analysis making it more useful for crime scenes that did not have the large amounts of DNA material that was previously required. [ 12 ]
Before modern PCR methods existed, it was almost impossible to analyze degraded DNA samples. Methods like restriction fragment length polymorphism (RFLP), which was the first technique used for DNA analysis in forensic science, required high molecular weight DNA in the sample in order to get reliable data.
RFLP is still used in marker-assisted selection. Terminal restriction fragment length polymorphism (TRFLP or sometimes T-RFLP) is a technique initially developed for characterizing bacterial communities in mixed-species samples. The technique has also been applied to other groups including soil fungi.
Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by Pieter Vos, [ 1 ] AFLP uses restriction enzymes to digest genomic DNA , followed by ligation of adaptors to the sticky ends of the restriction ...
Simple Sequence Length Polymorphisms (SSLPs) are used as genetic markers with polymerase chain reaction (PCR). An SSLP is a type of polymorphism: a difference in DNA sequence amongst individuals. SSLPs are repeated sequences over varying base lengths in intergenic regions of deoxyribonucleic acid (DNA).
Ancestry informative markers have a number of applications in genetic research, forensics, and private industry. AIMs that indicate a predisposition for diseases such as type 2 diabetes mellitus and renal disease have been shown to reduce the effects of genetic admixture in ancestral mapping when using admixture mapping software. [10]
In forensic science, hybridization probes are used, for example, for detection of short tandem repeats (microsatellite) regions [9] and in restriction fragment length polymorphism methods, all of which are widely used as part of DNA profiling analysis.