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Small acid-soluble proteins (SASPs) are found in endospores. These proteins tightly bind and condense the DNA, and are in part responsible for resistance to UV light and DNA-damaging chemicals. [3] Visualising endospores under light microscopy can be difficult due to the impermeability of the endospore wall to dyes and stains. While the rest of ...
[1] [2] Once stained as part of a sample, these organisms can resist the acid and/or ethanol-based decolorization procedures common in many staining protocols, hence the name acid-fast. [ 2 ] The mechanisms of acid-fastness vary by species although the most well-known example is in the genus Mycobacterium , which includes the species ...
Endospore stain on Bacillus subtilis.The spore is stained green and the vegetative cell is stained a pinkish red color. Endospore staining is a technique used in bacteriology to identify the presence of endospores in a bacterial sample. [1]
Dipicolinic acid composes 5% to 15% of the dry weight of Bacillus subtilis spores. [3] [4] It has been implicated as responsible for the heat resistance of the endospore, [3] [5] although mutants resistant to heat but lacking dipicolinic acid have been isolated, suggesting other mechanisms contributing to heat resistance are at work. [6]
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .
Acid pH 3: several species ... resistant, dormant structures called endospores. ... the cells are adapting to the high-nutrient environment and preparing for fast ...
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Dipicolinic acid is a chemical compound which composes 5% to 15% of the dry weight of bacterial spores and is implicated in being responsible for the heat resistance of endospores. Archaeologists have found viable endospores taken from the intestines of Egyptian mummies as well as from lake sediments in Northern Sweden estimated to be many ...