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MAPseq or Multiplexed Analysis of Projections by Sequencing is a RNA-Seq based method for high-throughput mapping of neuronal projections. It was developed by Anthony M. Zador and his team at Cold Spring Harbor Laboratory and published in Neuron, a Cell Press magazine.
The Expression Atlas allows searches by gene, splice variant, protein attribute, disease, treatment or organism part (cell types/tissues). Individual genes or gene sets can be searched for. All datasets in Expression Atlas have its metadata manually curated and its data analysed through standardised analysis pipelines.
A list of more than 100 different single cell sequencing (omics) methods have been published. [1] The large majority of methods are paired with short-read sequencing technologies, although some of them are compatible with long read sequencing.
Regev compared scientific knowledge of cell biology before the Human Cell Atlas initiative with a “15 th century map.” “Now, years later, the resolution of the map is a lot higher,” she said.
Unveiled in July 2008, the Allen Mouse Spinal Cord Atlas was the first genome-wide map of the mouse spinal cord ever constructed. The spinal cord atlas is a map of genome wide gene expression in the spinal cord of adult and juvenile C57 black mice. The initial unveiling included data for 2,000 genes and an anatomical reference section.
Single-cell RNA sequencing (scRNA-seq) provides the expression profiles of individual cells and is considered the gold standard for defining cell states and phenotypes as of 2020. [44] Although it is impossible to obtain complete information on every RNA expressed by each cell, due to the small amount of material available, gene expression ...
Among the products of the program is the Azimuth reference datasets for single-cell RNA seq data [2] [3] and the ASCT+B Reporter, a visualization tool for anatomical structures, cell types and biomarkers. [4] [5] Millitomes are used to create uniformly sized tissue blocks that match the shape and size of organs from HuBMAP's 3D Reference Object ...
Optical mapping [1] is a technique for constructing ordered, genome-wide, high-resolution restriction maps from single, stained molecules of DNA, called "optical maps". By mapping the location of restriction enzyme sites along the unknown DNA of an organism, the spectrum of resulting DNA fragments collectively serves as a unique "fingerprint" or "barcode" for that sequence.