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Osazone formation was developed by Emil Fischer, [3] who used the reaction as a test to identify monosaccharides. The formation of a pair of hydrazone functionalities involves both oxidation and condensation reactions. [4] Since the reaction requires a free carbonyl group, only "reducing sugars" participate.
The ICUMSA Methods Book [3] contains detailed instructions for analyzing raw, cane, white, beet, molasses, plantation white and specialty sugars. Among these are methods for determination of dry solids content by polarimetry, densimetry and refractometry, color (extinction coefficient at 420 nm), reducing sugars, and the presence of metals such ...
[7] [8] Hydrazone-based coupling methods are used in medical biotechnology to couple drugs to targeted antibodies (see ADC), e.g. antibodies against a certain type of cancer cell. The hydrazone-based bond is stable at neutral pH (in the blood), but is rapidly destroyed in the acidic environment of lysosomes of the cell.
An example of a positive Seliwanoff’s test. Seliwanoff’s test is a chemical test which distinguishes between aldose and ketose sugars. If the sugar contains a ketone group, it is a ketose. If a sugar contains an aldehyde group, it is an aldose. This test relies on the principle that, when heated, ketoses are more rapidly dehydrated than ...
The chemical complexity of coffee is emerging, especially due to observed physiological effects which cannot be related only to the presence of caffeine. Moreover, coffee contains an exceptionally substantial amount of antioxidants such as chlorogenic acids, hydroxycinnamic acids, caffeine and Maillard reaction products, such as melanoidins. [3]
Saccharin, also called saccharine, benzosulfimide, or E954, or used in saccharin sodium or saccharin calcium forms, is a non-nutritive artificial sweetener. [1] [5] Saccharin is a sultam that is about 500 times sweeter than sucrose, but has a bitter or metallic aftertaste, especially at high concentrations. [1]
Barfoed's test is a chemical test used for detecting the presence of monosaccharides. It is based on the reduction of copper(II) acetate to copper(I) oxide (Cu 2 O), which forms a brick-red precipitate. [1] [2] RCHO + 2Cu 2+ + 2H 2 O → RCOOH + Cu 2 O↓ + 4H + (Disaccharides may also react, but the reaction is much slower.)
The flame test is a more well known version of the chemical test. It is only used on metallic ions. The metal powder is burned, causing an emission of colors based on what metal was burned. For example, calcium (Ca) will burn orange and copper (Cu) will burn blue. Their color emissions are used to produce bright colors in fireworks. [citation ...