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Move the pipette to the receiving vessel and dispense the liquid by gently pressing the pipette knob to the first stop. After about one second, keep on pressing pipette knob to the second stop. This action will completely empty the tip from the liquid. Afterwards withdraw the tip from the liquid and release the pipette to the ready position.
Depending on the number of pistons in a pipette, there is a differentiation between single-channel pipettes and multi-channel pipettes. For manual high-throughput applications like filling up a 96-well microtiter plate most researchers prefer a multi-channel pipette. Instead of handling well by well, a row of 8 wells can be handled in parallel ...
Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or electronic pipettes. Many pipette types work by creating a partial vacuum above the liquid-holding chamber and selectively releasing this vacuum to draw up and dispense liquid ...
(These are for Class A pipettes; Class B pipettes are given a tolerance of twice that for the corresponding Class A.) A specialized example of a volumetric pipette is the microfluid pipette (capable of dispensing as little as 10 μL) designed with a circulating liquid tip that generates a self-confining volume in front of its outlet channels. [3]
A small pipette allows for more precise measurement of fluids; a larger pipette can be used to measure volumes when the accuracy of the measurement is less critical. Accordingly, pipettes vary in volume, with most measuring between 0 and 25.0 millilitres (0.00 and 0.88 imp fl oz; 0.00 and 0.85 US fl oz). [2]
A Mohr pipette, also known as a graduated pipette, is a type of pipette used to measure the volume of the liquid dispensed, although not as accurately as a volumetric pipette. [1] These use a series of marked lines (as on a graduated cylinder) to indicate the different volumes. [ 2 ]
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