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A multifocal diffractive lens is a diffractive optical element (DOE) that allows a single incident beam to be focused simultaneously at several positions along the propagation axis. [ 1 ] Example of multifocal peak intensity distribution along optical axis.(Courtesy of Holo/Or) Intensity distribution of multifocal lens 5 foci in Z-X plane
In a digital camera, diffraction effects interact with the effects of the regular pixel grid. The combined effect of the different parts of an optical system is determined by the convolution of the point spread functions (PSF). The point spread function of a diffraction limited circular-aperture lens is simply the Airy disk. The point spread ...
Diagram illustrating near-field optics, with the diffraction of light coming from NSOM fiber probe, showing wavelength of light and the near-field. [1] Comparison of photoluminescence maps recorded from a molybdenum disulfide flake using NSOM with a campanile probe (top) and conventional confocal microscopy (bottom). Scale bars: 1 μm. [2]
To understand the process, it is helpful to understand interference and diffraction. Interference occurs when one or more wavefronts are superimposed. Diffraction occurs when a wavefront encounters an object. The process of producing a holographic reconstruction is explained below purely in terms of interference and diffraction.
A bright-field microscope has many important parts including; the condenser, the objective lens, the ocular lens, the diaphragm, and the aperture. Some other pieces of the microscope that are commonly known are the arm, the head, the illuminator, the base, the stage, the adjusters, and the brightness adjuster.
The mathematical function which describes a Bessel beam is a solution of Bessel's differential equation, which itself arises from separable solutions to Laplace's equation and the Helmholtz equation in cylindrical coordinates. The fundamental zero-order Bessel beam has an amplitude maximum at the origin, while a high-order Bessel beam (HOBB ...
A lens or telescope is then used to form images of the original slit, with images formed using different wavelengths of light at different positions. If a real image is formed, it can be recorded on film or an image sensor, making the device a spectrograph. Replacing the prism with a diffraction grating would result in a grating spectrometer ...
Diffraction of light on the sample slide can spread the fluorescence signal and result in blurring in the convoluted images. Similarly, if there is a misalignment between the objective lens, filter, and detector, the excitation or emission beam may not be in focus and can cause blurring in the images. [14]