Search results
Results from the WOW.Com Content Network
Iodoacetamide (IAA) is an organic compound with the chemical formula I C H 2 CO NH 2. It is an alkylating agent used for peptide mapping purposes. Its actions are similar to those of iodoacetate .
[5] [10] [16] [17] For the quantitative and homogeneous alkylation of cysteines the position of the modification step in the sample-preparation process is crucial. With denaturing electrophoresis it is strongly recommended to perform the reaction before the execution of the electrophoresis, since there are free acrylamide monomers in the gel ...
Iodoacetate is an irreversible inhibitor of all cysteine peptidases, with the mechanism of inhibition occurring from alkylation of the catalytic cysteine residue (see schematic). In comparison with its amide derivative, iodoacetamide, iodoacetate reacts substantially slower. This observation appears contradictory to standard chemical reactivity ...
Alkylation is a chemical reaction that entails transfer of an alkyl group. The alkyl group may be transferred as an alkyl carbocation , a free radical , a carbanion , or a carbene (or their equivalents).
It is traditionally divided to a normal mass shift (NMS) resulting from the change in the reduced electronic mass, and a specific mass shift (SMS), which is present in multi-electron atoms and ions. The NMS is a purely kinematical effect, studied theoretically by Hughes and Eckart. [ 3 ]
An isotope-coded affinity tag (ICAT) is an in-vitro isotopic labeling method used for quantitative proteomics by mass spectrometry that uses chemical labeling reagents. [1] [2] [3] These chemical probes consist of three elements: a reactive group for labeling an amino acid side chain (e.g., iodoacetamide to modify cysteine residues), an isotopically coded linker, and a tag (e.g., biotin) for ...
Molar mass: 125.12528 Melting point: 43 to 46 °C (109 to 115 °F; 316 to 319 K) ... with alkylation occurring at the active site thiol group (see schematic). [3] [4]
Molar mass: 93.51 g·mol −1 Appearance Colorless crystals or white fine powder [1] (yellow if impure) Odor: Characteristic ... Iodoacetamide; Chloroacetic acid;