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Different dialysis tubing or flat membranes are produced and characterized as differing molecular-weight cutoffs (MWCO) ranging from 1–1,000,000 kDa. The MWCO determination is the result of the number and average size of the pores created during the production of the dialysis membrane.
In ultrafiltration, the molecular weight cut-off or MWCO of a membrane refers to the lowest molecular weight of the solute (in daltons) for which 90% of the solute is retained by (prevented from passing through) the membrane, [1] or the molecular weight of the molecule (e.g. globular protein) that is 90% retained by the membrane.
Dialysis tubing is the oldest and generally the least expensive format used for dialysis in the lab. Tubing is cut and sealed with a clip at one end, then filled and sealed with a clip on the other end. Tubing provides flexibility but has increased concerns regarding handling, sealing and sample recovery. Dialysis tubing is typically supplied ...
Hemodialysis, also spelled haemodialysis, or simply dialysis, is a process of filtering the blood of a person whose kidneys are not working normally. This type of dialysis achieves the extracorporeal removal of waste products such as creatinine and urea and free water from the blood when the kidneys are in a state of kidney failure.
Schematic of semipermeable membrane during hemodialysis, where blood is red, dialysing fluid is blue, and the membrane is yellow. Kidney dialysis (from Greek διάλυσις, dialysis, 'dissolution'; from διά, dia, 'through', and λύσις, lysis, 'loosening or splitting') is the process of removing excess water, solutes, and toxins from the blood in people whose kidneys can no longer ...
Hemofiltration is sometimes used in combination with hemodialysis, when it is termed hemodiafiltration. Blood is pumped through the blood compartment of a high flux dialyzer, and a high rate of ultrafiltration is used, so there is a high rate of movement of water and solutes from blood to dialysate that must be replaced by substitution fluid that is infused directly into the blood line.
Molecules that are significantly smaller than the MWCO penetrate into the pores of the resin, while molecules larger than the MWCO are unable to enter the pores and remain together in the void volume of the column. By passing samples through a column resin bed with sufficient length and volume, macromolecules can be fully separated from small ...
In cell biology, diffusion is a main form of transport for necessary materials such as amino acids within cells. [1] Diffusion of solvents, such as water, through a semipermeable membrane is classified as osmosis.
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