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Bile Esculin Agar (BEA) is a selective differential agar used to isolate and identify members of the genus Enterococcus, [1] formerly part of the "group D streptococci" (enterococci were reclassified in their own genus in 1984).
Most strains are gamma-hemolytic (non-hemolytic), but some also display alpha-hemolytic activity on sheep blood agar plates. Strep bovis is a non-enterococci. [citation needed] Biochemical Tests mannitol salt: negative bile esculin: negative MR/VP: positive/negative nutrient gelatin: negative starch: positive DNase: negative [citation needed]
Bile esculin agar is used for the isolation of Enterococcus and group D Streptococcus species. CLED agar – cysteine, lactose, electrolyte-deficient agar is used to isolate and differentiate urinary tract bacteria, since it inhibits Proteus species swarming and can distinguish between lactose fermenters and nonfermenters.
The bacterium is catalase-negative and LAP-positive (like all streptococci), PYR-test and CAMP-test-positive, does not hydrolyze sodium hippurate, and does not grow in bile esculin agar. [21] It does not express any of the known Lancefield antigens .
An indole butyrate disc is used to differentiate between Neisseria gonorrhoeae (negative result) and Moraxella catarrhalis (positive result). This test involves a butyrate disk, which when smeared with a culture, will change color for a positive result after 5 minutes of incubation. A blue color is the result of a positive test. [29]
Aesculin is incorporated into agar with ferric citrate and bile salts (bile aesculin agar). [5] Hydrolysis of aesculin forms aesculetin (6,7-dihydroxycoumarin) and glucose. Aesculetin forms dark brown or black complexes with ferric citrate, allowing the test to be read. The bile aesculin agar is streaked and incubated at 37 °C (99 °F) for 24 ...
When inoculated with a sample organism and allowed to incubate, the medium will change color from red to yellow to indicate that the mannite has been fermented.
The NYC (New York City) medium or GC (Neisseria gonorrhoeae) medium agar is a type of selective media used for isolating Gonococci and N. meningitidis. [ 1 ] The growth of Neisseria gonorrhoeae colonies on New York City medium agar