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Cryogenic electron tomography (cryoET) is an imaging technique used to reconstruct high-resolution (~1–4 nm) three-dimensional volumes of samples, often (but not limited to) biological macromolecules and cells.
CryoTEM image of GroEL suspended in amorphous ice at 50 000 × magnification Structure of Alcohol oxidase from Pichia pastoris by CryoTEM. Transmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures (generally liquid ...
Cryogenic transmission electron microscopy (cryo-TEM) is a transmission electron microscopy technique that is used in structural biology and materials science. Colloquially, the term "cryogenic electron microscopy" or its shortening "cryo-EM" refers to cryogenic transmission electron microscopy by default, as the vast majority of cryo-EM is ...
Scanning electron cryomicroscopy (CryoSEM) is a form of electron microscopy where a hydrated but cryogenically fixed sample is imaged on a scanning electron microscope's cold stage in a cryogenic chamber. The cooling is usually achieved with liquid nitrogen. [1]
Electron tomography is an extension of traditional transmission electron microscopy and uses a transmission electron microscope to collect the data. In the process, a beam of electrons is passed through the sample at incremental degrees of rotation around the center of the target sample.
Most university research groups, for example, require permission to use such high-end electron microscopes at national lab facilities, which requires excessive time commitment. Universal challenges mainly involve becoming accustomed to the programming language desired and writing software that can tackle the very specific problems for a given ...
Cryotherapy is so hot...or cold right now.
Computed tomography (CT) and positron emission tomography (PET) scans are similar to MRI, but rely on different imaging techniques (X-rays and ionizing radiation, respectively). A variation of CT known as contrast CT also requires the subject to take in a contrast medium called a radiocontrast (typically by oral consumption, enema, or injection).