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Giemsa's solution is a mixture of methylene blue, eosin, and Azure B.The stain is usually prepared from commercially available Giemsa powder. A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide.
The most influential 1904 work. Giemsa was born in Hamburg to Gustav, a mining official and Franziska. He studied pharmacy and mineralogy at the University of Leipzig (1892-94), and then worked between 1895 and 1898 as a pharmacist at the government hospital in Dar-es-Salaam, German East Africa.
Brugia is a genus for a group of small roundworms. They are among roundworms that cause the parasitic disease filariasis. [1] Specifically, of the three species known, Brugia malayi and Brugia timori cause lymphatic filariasis in humans; and Brugia pahangi and Brugia patei infect domestic cats, dogs and other animals.
This stain develops varying colors for all cell structures (“Romanowsky-Giemsa effect) and thus was used in staining neutrophil polymorphs and cell nuclei. Common variants include Wright's stain, Jenner's stain, May-Grunwald stain, Leishman stain and Giemsa stain. All are used to examine blood or bone marrow samples.
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Routine analysis of blood in medical laboratories is usually performed on blood films stained with Romanowsky stains such as Wright's stain, Giemsa stain, or Diff-Quik. Wright-Giemsa combination stain is also a popular choice. These stains allow for the detection of white blood cell, red blood cell, and platelet abnormalities.
Brugia timori is a filarial (arthropod-borne) nematode (roundworm) which causes the disease "Timor filariasis", or "Timorian filariasis".While this disease was first described in 1965, [1] the identity of Brugia timori as the causative agent was not known until 1977. [2]
Blood film stained with Giemsa showing Plasmodium (center of image), the parasite that causes malaria infections.. In 1891 Romanowsky [8] [9] [10] developed a stain using a mixture of eosin (typically eosin Y) and aged solutions of methylene blue that formed hues unattributable to the staining components alone: distinctive shades of purple in the chromatin of the cell nucleus and within ...