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The Kastle–Meyer test is a presumptive blood test, first described in 1903, in which the chemical indicator phenolphthalein is used to detect the possible presence of hemoglobin. It relies on the peroxidase -like activity of hemoglobin in blood to catalyze the oxidation of phenolphthalin (the colorless reduced form of phenolphthalein) into ...
Another common presumptive test is the Kastle-Meyer or Phenolphthalein test. This is a catalytic test that detects the heme group in blood that transports oxygen and carbon dioxide. [ 5 ] A sterile cotton swab is soaked in distilled water and applied to the area of suspected blood to pick up some of the sample. [ 5 ]
Most people who take a drug test take a presumptive test, cheaper and faster than other methods of testing. However, it is less accurate and can render false results. The FDA recommends for confirmatory testing to be conducted and the placing of a warning label on the presumptive drug test: "This assay provides only a preliminary result.
Clinistrips quantitatively test for sugar in urine; The Kastle-Meyer test tests for the presence of hemoglobin; Salicylate testing is a category of drug testing that is focused on detecting salicylates such as acetylsalicylic acid for either biochemical or medical purposes. The Phadebas test tests for the presence of saliva for forensic purposes
The Kastle Meyer (KM) reagent is actually already basic in pH -- it is when the reduced phenolphthalein in the reagent is oxidized to phenolphthalein by the presence of heme (or some other oxidizer) that it is the phenolphthalein you are familiar with, which indeed, will turn pink in the presence of its alkaline solvent.
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If the sample contains hemoglobin, it will turn pink immediately upon addition of the peroxide, because of the generation of phenolphthalein. A positive test indicates the sample contains hemoglobin and, therefore, is likely blood. A false positive can result from the presence of substances with catalytic activity similar to hemoglobin.
The strong programme or strong sociology is a variety of the sociology of scientific knowledge (SSK) particularly associated with David Bloor, [1] Barry Barnes, Harry Collins, Donald A. MacKenzie, [2] and John Henry. The strong programme's influence on science and technology studies is credited as being unparalleled (Latour 1999).