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If A549 cells are cultured in vitro, they grow as a monolayer; adherent or attaching to the culture flask. [1] The cells are able to synthesize lecithin and contain high levels of unsaturated fatty acids, which are important to maintain membrane phospholipids. [1] A549 cells are widely used as a type II pulmonary epithelial cell model for drug ...
Immortalised cell lines are widely used as a simple model for more complex biological systems – for example, for the analysis of the biochemistry and cell biology of mammalian (including human) cells. [2] The main advantage of using an immortal cell line for research is its immortality; the cells can be grown indefinitely in culture.
HeLa cells are rapidly dividing cancer cells, and the number of chromosomes varies during cancer formation and cell culture. The current estimate (excluding very tiny fragments) is a "hypertriploid chromosome number (3n+)", which means 76 to 80 total chromosomes (rather than the normal diploid number of 46) with 22–25 clonally abnormal ...
A standard culture protocol with a tissue carrier can be initiated by seeding cells onto the upper side. When a tissue carrier is turned, cells can also be seeded on the other side so that co-culture experiments with two different cell types become possible.
Current Protocols is a series of laboratory manuals for life scientists. The first title, Current Protocols in Molecular Biology, was established in 1987 by the founding editors Frederick M. Ausubel, Roger Brent, Robert Kingston, David Moore, Jon Seidman, Kevin Struhl, and John A. Smith of the Massachusetts General Hospital Department of Molecular Biology and the Harvard Medical School ...
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
Download QR code; Print/export Download as PDF; Printable version; In other projects ... move to sidebar hide. A549 may refer to: A549 (cell line ), a carcinomic ...
Cell synchronization is a process by which cells in a culture at different stages of the cell cycle are brought to the same phase. Cell synchrony is a vital process in the study of cells progressing through the cell cycle as it allows population-wide data to be collected rather than relying solely on single-cell experiments.