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The intraoperative consultation is the name given to the whole intervention by the pathologist, which includes not only frozen section but also gross evaluation of the specimen, examination of cytology preparations taken on the specimen (e.g. touch imprints), and aliquoting of the specimen for special studies (e.g. molecular pathology ...
Typical specimens for cryofixation include small samples of plant or animal tissue, cell suspensions of microorganisms or cultured cells, suspensions of viruses or virus capsids and samples of purified macromolecules, especially proteins. [2] [3] Types of cryo-fixation are freezing-drying, freezing-substitution and freezing-etching.
Controlled-rate and slow freezing, also known as slow programmable freezing (SPF), [18] is a technique where cells are cooled to around -196 °C over the course of several hours. Slow programmable freezing was developed during the early 1970s, and eventually resulted in the first human frozen embryo birth in 1984. Since then, machines that ...
Technicians preparing a body for cryopreservation in 1985. Cryonics (from Greek: κρύος kryos, meaning "cold") is the low-temperature freezing (usually at −196 °C or −320.8 °F or 77.1 K) and storage of human remains in the hope that resurrection may be possible in the future.
Similar to the frozen section procedure employed in medicine, cryosectioning is a method to rapidly freeze, cut, and mount sections of tissue for histology. The tissue is usually sectioned on a cryostat or freezing microtome. [12] The frozen sections are mounted on a glass slide and may be stained to enhance the contrast between different tissues.
Cryotherapy, sometimes known as cold therapy, is the local or general use of low temperatures in medical therapy.Cryotherapy can be used in many ways, including whole body exposure for therapeutic health benefits or may be used locally to treat a variety of tissue lesions.
Low temperatures may be maintained within a cryostat by using various refrigeration methods, most commonly using cryogenic fluid bath such as liquid helium. [1] Hence it is usually assembled into a vessel, similar in construction to a vacuum flask or Dewar. Cryostats have numerous applications within science, engineering, and medicine.
At least six major areas of cryobiology can be identified: 1) study of cold-adaptation of microorganisms, plants (cold hardiness), and animals, both invertebrates and vertebrates (including hibernation), 2) cryopreservation of cells, tissues, gametes, and embryos of animal and human origin for (medical) purposes of long-term storage by cooling to temperatures below the freezing point of water.