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Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals) which catalyzes the decomposition of hydrogen peroxide to water and oxygen. [5] It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS).
In medical sterilization, hydrogen peroxide is used at higher concentrations, ranging from around 35% up to 90%. The biggest advantage of hydrogen peroxide as a sterilant is the short cycle time. Whereas the cycle time for ethylene oxide may be 10 to 15 hours, some modern hydrogen peroxide sterilizers have a cycle time as short as 28 minutes. [44]
The superoxide is converted to hydrogen peroxide and singlet oxygen by an enzyme called superoxide dismutase. Superoxides also react with the hydrogen peroxide to produce hydroxyl radicals, which assist in killing the invading microbe. [14] The second type involves the use of the enzyme myeloperoxidase from neutrophil granules. [34]
Superoxide can be produced in phagosomes which have ingested bacteria and fungi, or it can be produced outside of the cell. [10] In macrophages, superoxide kills bacteria and fungi by mechanisms that are not yet fully understood. [11] [12] Superoxide spontaneously dismutates to form peroxide which is then protonated to produce hydrogen peroxide ...
A disinfectant is a chemical substance or compound used to inactivate or destroy microorganisms on inert surfaces. [1] Disinfection does not necessarily kill all microorganisms, especially resistant bacterial spores; it is less effective than sterilization, which is an extreme physical or chemical process that kills all types of life. [1]
Phagocytosis (from Ancient Greek φαγεῖν (phagein) 'to eat' and κύτος (kytos) 'cell') is the process by which a cell uses its plasma membrane to engulf a large particle (≥ 0.5 μm), giving rise to an internal compartment called the phagosome. It is one type of endocytosis. A cell that performs phagocytosis is called a phagocyte.
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