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In 1952, Hershey and Chase [19] provided key evidence that the phage DNA, as distinct from protein, enters the host bacterial cell upon infection and is thus the genetic material of the phage. This finding suggested that DNA is, in general, the genetic material of different organisms.
Structural model at atomic resolution of bacteriophage T4 [1] The structure of a typical myovirus bacteriophage Anatomy and infection cycle of bacteriophage T4.. A bacteriophage (/ b æ k ˈ t ɪər i oʊ f eɪ dʒ /), also known informally as a phage (/ ˈ f eɪ dʒ /), is a virus that infects and replicates within bacteria and archaea.
The T4 rII system is an experimental system developed in the 1950s by Seymour Benzer for studying the substructure of the gene. The experimental system is based on genetic crosses of different mutant strains of bacteriophage T4 , a virus that infects the bacteria Escherichia coli .
Hershey and Chase concluded that DNA, not protein, was the genetic material. They determined that a protective protein coat was formed around the bacteriophage, but that the internal DNA is what conferred its ability to produce progeny inside a bacterium. They showed that, in growth, protein has no function, while DNA has some function.
The binding is due to electrostatic interactions and is influenced by pH and the presence of ions. The virus then releases its genetic material (either single- or double-stranded RNA or DNA) into the cell. In some viruses this genetic material is circular and mimics a bacterial plasmid. At this stage the cell becomes infected and can also be ...
The genetic material of the bacteriophage, called a prophage, can be transmitted to daughter cells at each subsequent cell division, and later events (such as UV radiation or the presence of certain chemicals) can release it, causing proliferation of new phages via the lytic cycle. [1]
These experiments, carried out with mutants of the rIIB gene of phage T4, showed, that for a gene that encodes a protein, three sequential bases of the gene's DNA specify each successive amino acid of the protein. Thus the genetic code is a triplet code, where each triplet (called a codon) specifies a particular amino acid.
A capsid is the protein shell of a virus, enclosing its genetic material. ... The bacterium E. coli is the host for bacteriophage T4 that has a prolate head structure.