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Authentication should be repeated before freezing cell line stocks, every two months during active culturing and before any publication of research data generated using the cell lines. Many methods are used to identify cell lines, including isoenzyme analysis, human lymphocyte antigen (HLA) typing, chromosomal analysis, karyotyping, morphology ...
This is the original method for generating an immortalised cell line. A major example is human HeLa , a line derived from cervical cancer cells taken on February 8, 1951 from Henrietta Lacks, a 31-year-old African-American mother of five, who died of cancer on October 4, 1951.
MRC-5 (Medical Research Council cell strain 5) is a diploid cell culture line composed of fibroblasts, originally developed from the lung tissue of a 14-week-old aborted white male fetus. [ 1 ] [ 2 ] The cell line was isolated by J.P. Jacobs and colleagues in September 1966 from the seventh population doubling of the original strain, and MRC-5 ...
Since many cell lines used in research have been misidentified or contaminated, the Cellosaurus keeps track of problematic cell lines, including all those listed in the International Cell Line Authentication Committee (ICLAC) [8] tables. For human as well as some dog cell lines, it provides short tandem repeat (STR) profile
[3] [61] The International Cell Line Authentication Committee (ICLAC) notes that many cases of cell line misidentification are the result of cross-contamination of the culture by another, faster-growing cell line. This calls into question the validity of the research done using contaminated cell lines, as certain attributes of the contaminant ...
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The International Cell Line Authentication Committee maintains a list of contaminated cell lines. [10] It includes the cell lines reported above, although usually with reporting dates and articles considerably later than the first dates and articles rising specific concern regarding the respective cell line.