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Swarming motility is a rapid (2–10 μm/s) and coordinated translocation of a bacterial population across solid or semi-solid surfaces, [1] and is an example of bacterial multicellularity and swarm behaviour.
The vertical growth or elevation of the colony, another identifying characteristic, is assessed by tilting the agar plate to the side and is denoted as flat, raised, convex, pulvinate (very convex), umbilicate (having a depression in the centre) or umbonate (having a bump in the centre).
The swarming capability of Proteus mirabilis is important because it is implicated in the pathogenesis of the bacteria and the swarming capability is associated with the bacteria's ability to express virulence factors [9] Proteus mirabilis has a very characteristic bulls-eye appearance on an agar plate due to the regular periodic cycling ...
Swarming motility is a rapid (2–10 μm/s) and coordinated translocation of a bacterial population across solid or semi-solid surfaces, [61] and is an example of bacterial multicellularity and swarm behaviour. Swarming motility was first reported in 1972 by Jorgen Henrichsen.
Strains possessing one or both of these hemolysins exhibit beta-hemolysis on blood agar plates. A distinct correlation seems to exist between presence of tdh, trh, and the two known T3SS variants: observations have shown T3SS2α correlating with tdh+/trh- strains, while T3SS2β correlates with tdh-/trh+ strains. [7]
Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
The optimum growth condition for P. penneri is achieved at 37 °C, which mirrors the intestinal niche colonized by many of these bacteria. Certain strains of P. penneri can differentiate into elongated hyperflagelled cells during development on solid media, resulting in the surface translocation event identified as “swarming”.
M. morganii grown on blood agar. Morganella morganii is facultatively anaerobic and oxidase-negative. Its colonies appear off-white and opaque in color, when grown on agar plates. [7] M. morganii cells are straight rods, about 0.6–0.7 μm in diameter and 1.0–1.7 μm in length.