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A Basophil granulocyte stains dark purple upon H&E staining. Basophilic is a technical term used by pathologists. It describes the appearance of cells, tissues and cellular structures as seen through the microscope after a histological section has been stained with a basic dye. The most common such dye is haematoxylin.
Basophils are the least common type of granulocyte, representing about 0.5% to 1% of circulating white blood cells. [1] They are the largest type of granulocyte. They are responsible for inflammatory reactions during immune response, as well as in the formation of acute and chronic allergic diseases, including anaphylaxis , asthma , atopic ...
There are four types of granulocytes (full name polymorphonuclear granulocytes): [3] Basophils; Eosinophils; Neutrophils; Mast cells; Except for the mast cells, their names are derived from their staining characteristics; for example, the most abundant granulocyte is the neutrophil granulocyte, which has neutrally staining cytoplasmic granules.
An anterior pituitary basophil is a type of cell in the anterior pituitary which manufactures hormones. It is called a basophil because it is basophilic (readily takes up bases), and typically stains a relatively deep blue or purple. [1] These basophils are further classified by the hormones they produce.
By adding EDTA to the test tube, the degranulation process is stopped immediately. After degranulation a CD63 marker (labeled antibodies) is added to the test tube. Several minutes at room temperature gives the marker time to bind to the CD63 proteins on the cell membrane of the basophil. A lysing step is performed to lyse the red blood cells.
A white blood cell differential is a medical laboratory test that provides information about the types and amounts of white blood cells in a person's blood. The test, which is usually ordered as part of a complete blood count (CBC), measures the amounts of the five normal white blood cell types – neutrophils, lymphocytes, monocytes, eosinophils and basophils – as well as abnormal cell ...
Basopenia (or basocytopenia) is a form of agranulocytosis associated with a deficiency of basophils. [1] It has been proposed as an indicator of ovulation. [2] It is difficult to detect without flow cytometry, because normal levels are so low. [3]
Therefore, there are two different granule populations in gonadotropes, one type being 150-250 nm in diameter and the other being 350-450 nm in diameter. [4] Gonadotropes are usually described as globular and basophilic due to the cells’ ability to absorb dyes that appear blue or purple under the microscope due to the cytoplasmic granules ...
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