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At a wavelength of 260 nm, the average extinction coefficient for double-stranded DNA (dsDNA) is 0.020 (μg/mL) −1 cm −1, for single-stranded DNA (ssDNA) it is 0.027 (μg/mL) −1 cm −1, for single-stranded RNA (ssRNA) it is 0.025 (μg/mL) −1 cm −1 and for short single-stranded oligonucleotides it is dependent on the length and base ...
The nucleic acid notation currently in use was first formalized by the International Union of Pure and Applied Chemistry (IUPAC) in 1970. [1] This universally accepted notation uses the Roman characters G, C, A, and T, to represent the four nucleotides commonly found in deoxyribonucleic acids (DNA).
Cresyl violet stained partial brain section of a Macaque. It is used in biology and medicine as a histological stain. Cresyl violet is an effective and reliable stain used for light microscopy sections. Initially, tissue sections are "defatted" by passing through graded dilutions of ethanol. Then, rehydrated by passing back through decreasing ...
As the two strands of a double-stranded nucleic acid molecule are antiparallel, the 5′→3′ direction on the second strand corresponds to the 3′→5′ direction along the first strand. [1] [2] In general, at the most, one reading frame in a given section of a nucleic acid, is biologically relevant (open reading frame). Some viral ...
Nucleic acid NMR is the use of NMR spectroscopy to obtain information about the structure and dynamics of nucleic acid molecules, such as DNA or RNA. As of 2003, nearly half of all known RNA structures had been determined by NMR spectroscopy. [2] Nucleic acid NMR uses similar techniques as protein NMR, but has several differences.
Giemsa stained Trypanosoma parasites (Chagas disease pathogen) Whirling disease section stained with Giemsa stain. Giemsa stain (/ ˈ ɡ iː m z ə /), named after German chemist and bacteriologist Gustav Giemsa, is a nucleic acid stain used in cytogenetics and for the histopathological diagnosis of malaria and other parasites.
Nucleic acids present in the washed (and preferably dried) silica-nucleic acid complexes is eluted into chosen elution buffer such as TE buffer, aqua bidest, and so on. The selection of the elution buffer is co-determined by the contemplated use of the isolated nucleic acid. In this way, pure nucleic acids are isolated from the starting material.
The second generation, the Qubit 2.0 Fluorometer, was released in 2010, and the 3rd generation as Qubit 3.0 in 2014. The newest version is the 4th generation Qubit 4, introduced in 2017. References