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MALDI mass spectrometry imaging (MALDI-MSI) is the use of matrix-assisted laser desorption ionization as a mass spectrometry imaging [2] technique in which the sample, often a thin tissue section, is moved in two dimensions while the mass spectrum is recorded. [3]
The term matrix-assisted laser desorption ionization (MALDI) was coined in 1985 by Franz Hillenkamp, Michael Karas and their colleagues. [3] These researchers found that the amino acid alanine could be ionized more easily if it was mixed with the amino acid tryptophan and irradiated with a pulsed 266 nm laser.
The schematic of IR-MALDESI imaging source. The IR-MALDESI source can be used for mass spectrometry imaging (MSI), a technique using MS data analyzed from a sample area to detect hundreds to thousands of biomolecules and visualize their spatial distributions.
More than 50 years ago, MSI was introduced using secondary ion mass spectrometry (SIMS) to study semiconductor surfaces by Castaing and Slodzian. [5] However, it was the pioneering work of Richard Caprioli and colleagues in the late 1990s, demonstrating how matrix-assisted laser desorption/ionization (MALDI) could be applied to visualize large biomolecules (as proteins and lipids) in cells and ...
In mass spectrometry, a matrix is a compound that promotes the formation of ions. Matrix compounds are used in matrix-assisted laser desorption/ionization (MALDI), matrix-assisted ionization (MAI), and fast atom bombardment (FAB).
Mass spectrometric immunoassay (MSIA) is a rapid method is used to detect and/ or quantify antigens and or antibody analytes. [1] This method uses an analyte affinity (either through antigens or antibodies) isolation to extract targeted molecules and internal standards from biological fluid in preparation for matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI ...
MALDI is an ionization method used in mass spectrometry, allowing the analysis of large biopolymers. Although Karas and Hillenkamp were the first to discover MALDI, Japanese engineer Koichi Tanaka was the first to use a similar method in 1988 to ionize proteins [10] and shared the Nobel Prize in Chemistry in 2002 for that work. [11]
MS 2 – Mass spectrometry/mass spectrometry, i.e. tandem mass spectrometry; MS/MS – Mass spectrometry/mass spectrometry, i.e. tandem mass spectrometry; MALDESI – Matrix-assisted laser desorption electrospray ionization; MALDI – Matrix-assisted laser desorption/ionization; MAII – Matrix-assisted inlet ionization; MAIV – Matrix ...