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The AP-MALDI ion source is easily coupled to an ion trap mass spectrometer [34] or any other MS system equipped with electrospray ionization (ESI) or nanoESI source. MALDI with ionization at reduced pressure is known to produce mainly singly-charged ions (see "Ionization mechanism" below).
There are other hybrid ionization methods which combine resonant or non-resonant laser desorption with post electrospray ionization. One example is electrospray laser desorption ionization (ELDI), which uses an ultraviolet laser to form ions by irradiating the sample directly, then interacting with the electrospray plume without using any ...
Extractive electrospray ionization is a spray-type, ambient ionization method that uses two merged sprays, one of which is generated by electrospray. [ 49 ] Laser-based electrospray-based ambient ionization is a two-step process in which a pulsed laser is used to desorb or ablate material from a sample and the plume of material interacts with ...
The two primary methods for ionization of proteins are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). In electrospray, the ions are created from proteins in solution, and it allows fragile molecules to be ionized intact, sometimes preserving non-covalent interactions.
MS-LAMP is a standalone software capable of aiding in interpreting electrospray ionization (ESI) and/or matrix-assisted laser desorption and ionization (MALDI) mass spectrometric data of lipids. [9] MyriMatch Open source
Ions are typically formed by electrospray ionization, [3] but they can also be formed by matrix-assisted laser desorption/ionization [4] or other ionization techniques. It has applications in basic research in proteomics [ 5 ] and quantitative analysis of biomolecules [ 6 ] as well as in clinical medicine .
The DART ionization process can produce positive or negative ions depending on the potential applied to the exit electrode. This ionization can occur for species desorbed directly from surfaces such as bank notes, tablets, bodily fluids (blood, saliva and urine), polymers, glass, plant leaves, fruits & vegetables, clothing, and living organisms.
Schematic of LSII. Laserspray inlet ionization (LSII) is a subset of MAII and uses a matrix-assisted laser desorption/ionization method.It was originally called atmospheric pressure matrix-assisted laser desorption/ionization however was renamed as LSII to avoid confusion with MALDI and as it was found to be a type of inlet ionization.
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