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The term "vital stain" is used by some authors to refer specifically to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell being examined is still alive. As the cells are alive and unfixed, outside the body, supravital stains are temporary in nature. [1] [2]
The term vital stain is used by some authors to refer to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell being examined is still alive. In a more strict sense, the term vital staining has a meaning contrasting with supravital staining.
For example, tissues that stain with an azure stain may be referred to as azurophilic. This may also be used for more generalized staining properties, such as acidophilic for tissues that stain by acidic stains (most notably eosin ), basophilic when staining in basic dyes, and amphophilic [ 24 ] when staining with either acid or basic dyes.
Brilliant cresyl blue is a supravital stain used for counting reticulocytes. [2] It is classified as an oxazine dye. N95 dust masks, eye shields, and gloves must all be worn when handling the chemical. [3]
Reticulocytes appear slightly bluer than other red cells when looked at with the normal Romanowsky stain. Reticulocytes are also relatively large, a characteristic that is described by the mean corpuscular volume. Supravital stain of a smear of human blood from a patient with hemolytic anemia. The reticulocytes are the cells with the dark blue ...
Heinz body stain of feline blood, showing three distinct Heinz bodies. Heinz bodies appear as small round inclusions within the red cell body, though they are not visible when stained with Romanowsky dyes. They are visualized more clearly with supravital staining [5] [6] (e.g., with new methylene blue, crystal violet or bromocresol green).
Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900. [2] The indicator Janus Green B changes colour according to the amount of oxygen present. [3] When oxygen is present, the indicator oxidizes to a blue colour.
It is a supravital stain. [2] It is closely related to methylene blue, an older stain in wide use. Safety. New methylene blue is toxic. Skin contact or inhalation ...