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Colonies of Micrococcus luteus on Tryptic Soy Agar. Cultivation 48 hours, 37°C. Trypticase soy agar or Tryptic soy agar (TSA) is a growth media for the culturing of moderately to non fastidious bacteria. It is a general-purpose, non-selective media providing enough nutrients to allow for a wide variety of microorganisms to grow.
Brain heart infusion media made from Difco powdered media. Brain heart infusion (BHI) is a growth medium for growing microorganisms. It is a nutrient-rich medium, and can therefore be used to culture a variety of fastidious organisms.
Once a plate has been successfully prepared, plate count agar cells will grow into colonies which can be sufficiently isolated to determine the original cell type. The colony-forming unit (CFU) is an appropriate description of the colony's origin. In plate counts, colonies are counted, but the count is usually recorded in CFU.
Tryptic soy-serum-bacitracin-vancomycin (TSBV) is a type of agar plate medium used in microbiological testing to select for Aggregatibacter actinomycetemcomitans (A. a.). [1] It was described by Jørgen Slots in 1982, who also discovered the role of A.a. in periodontitis. [2] Per litre, TSBV contains: [3] 40 g tryptic soy agar; 1 g yeast extract
Eaton's agar is a type of agar media is used to grow Mycoplasma pneumoniae. One recipe for the cultivation of M. pneumoniae (Eaton's agar) includes : [1] 70% Difco PPLO (pleuropneumonia-like organism) agar or broth base; 20% unheated horse serum; 10% fresh aqueous extract of baker's yeast; 1000 units/ml Penicillin G
Tryptic soy broth or Trypticase soy broth (frequently abbreviated as TSB) is used in microbiology laboratories as a culture broth to grow aerobic and facultative anaerobic bacteria. It is a general purpose medium that is routinely used to grow bacteria which tend to have high nutritional requirements (i.e., they are fastidious ).
Aerobic and anaerobic bacteria can be identified by growing them in test tubes of thioglycolate broth: 1: Obligate aerobes need oxygen because they cannot ferment or respire anaerobically.
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