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ATP is a molecule found only in and around living cells, and as such it gives a direct measure of biological concentration and health. ATP is quantified by measuring the light produced through its reaction with the naturally-occurring firefly enzyme luciferase using a luminometer.
ATP quantification; Immunoassays [11] High throughput screening of compounds and targets in drug discovery (Labeled Alpha Screen on most instruments) [12] Bead-based epitope assay [13] Cellular Uptake of nanoparticles [14] While "plate reader" usually refers to the devices described above, many variations are available.
A specific instance of this technology is the Qubit 2.0 fluorometer, which is often used in conjunction with the "dsDNA BR Assay Kit." This kit, along with others in the Qubit quantification system, incorporates dyes. These dyes are sensitive to different biomolecules and their concentrations.
The adenylate energy charge is an index used to measure the energy status of biological cells.. ATP or Mg-ATP is the principal molecule for storing and transferring energy in the cell : it is used for biosynthetic pathways, maintenance of transmembrane gradients, movement, cell division, etc...
BCA protein assay in a 96 well plate. The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, [1] now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein assay or ...
Adenosine triphosphate (ATP) is a nucleoside triphosphate [2] that provides energy to drive and support many processes in living cells, such as muscle contraction, ...
The Bradford protein assay (also known as the Coomassie protein assay) was developed by Marion M. Bradford in 1976. [1] It is a quick and accurate [2] spectroscopic analytical procedure used to measure the concentration of protein in a solution.
The rate of a reaction is the concentration of substrate disappearing (or product produced) per unit time (mol L −1 s −1).. The % purity is 100% × (specific activity of enzyme sample / specific activity of pure enzyme).