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  2. Polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction

    A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.

  3. File:PCR diagrams.pdf - Wikipedia

    en.wikipedia.org/wiki/File:PCR_diagrams.pdf

    Original file (1,275 × 1,752 pixels, file size: 938 KB, MIME type: application/pdf, 3 pages) This is a file from the Wikimedia Commons . Information from its description page there is shown below.

  4. Touchdown polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Touchdown_polymerase_chain...

    The earliest steps of a touchdown polymerase chain reaction cycle have high annealing temperatures. The annealing temperature is decreased in increments for every subsequent set of cycles. The primer will anneal at the highest temperature which is least-permissive of nonspecific binding that it is able to tolerate.

  5. Reverse Transcription Loop-mediated Isothermal Amplification

    en.wikipedia.org/wiki/Reverse_Transcription_Loop...

    This cycle can be started from either the forward or backward side of the strand using the appropriate primer. Once this cycle has begun, the strand undergoes self-primed DNA synthesis during the elongation stage of the amplification process. This amplification takes place in less an hour, under isothermal conditions between 60 and 65 °C.

  6. Reverse transcription polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Reverse_transcription...

    Two-step RT-PCR, as the name implies, occurs in two steps. First the reverse transcription and then the PCR. This method is more sensitive than the one-step method. Kits are also useful for two-step RT-PCR. Just as for one-step PCR, use only intact, high-quality RNA for the best results. The primer for two-step PCR does not have to be sequence ...

  7. Sanger sequencing - Wikipedia

    en.wikipedia.org/wiki/Sanger_sequencing

    In contrast, PCR-based cloning and next-generation sequencing technologies based on pyrosequencing often avoid using cloning vectors. Recently, one-step Sanger sequencing (combined amplification and sequencing) methods such as Ampliseq and SeqSharp have been developed that allow rapid sequencing of target genes without cloning or prior ...

  8. COLD-PCR - Wikipedia

    en.wikipedia.org/wiki/COLD-PCR

    The DNA polymerase will extend complementary to the template DNA. Since the heteroduplex DNA is used as template, a larger proportion of minor variant DNA will be amplified and be available for subsequent rounds of PCR. There are two forms of COLD-PCR that have been developed to date. Full COLD-PCR and fast COLD-PCR. Full COLD-PCR versus Fast ...

  9. Template:Polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Template:Polymerase_chain...

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