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Two resolved peaks in a chromatogram. The theoretical plate height is given by = where L is the column length and N the number of theoretical plates. [5] The relation between plate number and peak width at the base is given by = ().
The response factor can be expressed on a molar, volume or mass [1] basis. Where the true amount of sample and standard are equal: = where A is the signal (e.g. peak area) and the subscript i indicates the sample and the subscript st indicates the standard. [2]
The fundamental resolution equation is derived as follows: For two closely spaced peaks, ω 1 = ω 2, and σ 1 = σ 2. so R s = (t r2 - t r1)/ω 2 = (t r2 - t r1)/4σ 2. Where t r1 and t r2 are the retention times of two separate peaks. Since N = [(t r2)/σ 2] 2, then σ = t r2 / N 1/2. Using substitution, R s = N 1/2 [(t r2 - t r1)/4t r2] = (N ...
The Purnell equation is an equation used in analytical chemistry to calculate the resolution R s between two peaks in a chromatogram. [1] [2] = (′ + ′) where R s is the resolution between the two peaks N 2 is the plate number of the second peak α is the separation factor between the two peaks k ' 2 is the retention factor of the second peak.
PeakFit is an automated nonlinear peak separation and analysis software package for scientists performing spectroscopy, chromatography and electrophoresis.. PeakFit automatically finds and fits up to 100 peaks to a data set, at a time, enabling users to characterize peaks and find the best equation that fits their data.
Chromatography software is called also Chromatography Data System. [1] It is located in the data station of the modern liquid, gas or supercritical fluid chromatographic systems. This is a dedicated software connected to an hardware interface within the chromatographic system, which serves as a central hub for collecting, analyzing, and ...
The method interpolates peaks between bracketing n-alkanes. The Kovats index of n-alkanes is 100 times their carbon number, e.g. the Kovats index of n-butane is 400. The Kovats index is dimensionless, unlike retention time or retention volume. For isothermal gas chromatography, the Kovats index is given by the equation:
OpenChrom is an open source software for the analysis and visualization of mass spectrometric and chromatographic data. [4] Its focus is to handle native data files from several mass spectrometry systems (e.g. GC/MS, LC/MS, Py-GC/MS, HPLC-MS), vendors like Agilent Technologies, Varian, Shimadzu, Thermo Fisher, PerkinElmer and others.