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Ultraviolet germicidal irradiation (UVGI) is a disinfection technique employing ultraviolet (UV) light, particularly UV-C (180–280 nm), to kill or inactivate microorganisms. UVGI primarily inactivates microbes by damaging their genetic material, thereby inhibiting their capacity to carry out vital functions.
A germicidal lamp (also known as disinfection lamp or sterilizer lamp) is an electric light that produces ultraviolet C (UVC) light. This short-wave ultraviolet light disrupts DNA base pairing , causing formation of pyrimidine dimers , and leads to the inactivation of bacteria , viruses , and protozoans .
•Infrared light source-do- •Ultraviolet light source-do- Inspissator: used to produce culture media for bacteriology that contain egg or serum, which coagulate on heating Tyndallizer: a process of sterilization from spore bearing bacteria; video link: Water bath: to heat things uniformly from all sides at a set temperature up to the boiling ...
Ultraviolet (UV) light induces the formation of covalent linkages on DNA and thereby prevents microbes from reproducing. Without reproduction, the microbes become far less dangerous. Germicidal UV-C light in the short wavelength range of 100–280 nm acts on thymine, one of the four base nucleotides in DNA.
UV rays can damage the DNA of living organisms by creating nucleic acid dimers. However, the damages are usually not important due to low penetration of UVs through living tissues. UV rays can be used, however, to inactivate viruses since virus particules are small and the UV rays can reach the genetic material, inducing the dimerisation of ...
In addition to air filters, cleanrooms can also use ultraviolet light to disinfect the air. [14] UV devices can be fitted into ceiling light fixtures and irradiate air, killing potentially infectious particulates , including 99.99 percent of airborne microbial and fungal contaminants. [ 15 ]
These lamps emit ultraviolet light with two peaks in the UVC band at 253.7 nm and 185 nm due to the mercury within the lamp, as well as some visible light. From 85% to 90% of the UV produced by these lamps is at 253.7 nm, whereas only 5–10% is at 185 nm. [38] The fused quartz tube passes the 253.7 nm radiation but blocks the 185 nm wavelength.
Pathogen reduction using riboflavin and UV light is a method by which infectious pathogens in blood for transfusion are inactivated by adding riboflavin and irradiating with UV light. [ 1 ] [ 2 ] [ 3 ] This method reduces the infectious levels of disease-causing agents that may be found in donated blood components, while still maintaining good ...