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  2. Acid-fastness - Wikipedia

    en.wikipedia.org/wiki/Acid-fastness

    The most common staining technique used to identify acid-fast bacteria is the Ziehl–Neelsen stain, in which the acid-fast species are stained bright red and stand out clearly against a blue background. Another method is the Kinyoun method, in which the

  3. Nocardia - Wikipedia

    en.wikipedia.org/wiki/Nocardia

    Nocardia as found on a brain biopsy.. Nocardia is a genus of weakly staining Gram-positive, catalase-positive, rod-shaped bacteria.It forms partially acid-fast beaded branching filaments (appearing similar to fungi, but being truly bacteria).

  4. Ziehl–Neelsen stain - Wikipedia

    en.wikipedia.org/wiki/Ziehl–Neelsen_stain

    After the Ziehl-Neelsen staining procedure using carbol fuchsin, acid-fast bacteria are observable as vivid red or pink rods set against a blue or green background, depending on the specific counterstain used, such as methylene blue or malachite green, respectively. Non-acid-fast bacteria and other cellular structures will be colored by the ...

  5. Gram stain - Wikipedia

    en.wikipedia.org/wiki/Gram_stain

    Some bacteria have cell walls which are particularly adept at retaining stains. These will appear positive by Gram stain even though they are not closely related to other gram-positive bacteria. These are called acid-fast bacteria, and can only be differentiated from other gram-positive bacteria by special staining procedures. [25]

  6. Staining - Wikipedia

    en.wikipedia.org/wiki/Staining

    A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .

  7. Mycobacterium fortuitum - Wikipedia

    en.wikipedia.org/wiki/Mycobacterium_fortuitum

    Gram-positive, nonmotile and acid-fast rods (1-3 μm x 0.2-0.4 μm). Sometimes long rods with occasional beaded or swollen cells having non-acid-fast ovoid bodies at one end. Colony characteristics. Smooth hemispheric colonies, usually off-white or cream colored. May be butyrous, waxy, multilobate and even rosette clustered (dilute inocula).

  8. Kinyoun stain - Wikipedia

    en.wikipedia.org/wiki/Kinyoun_stain

    The Kinyoun method can be modified as a weak acid fast stain, which uses 0.5–1.0% sulfuric acid instead of hydrochloric acid.The weak acid fast stain, in addition to staining Mycobacteria, will also stain organisms that are not able to maintain the carbol fuchsin after decolorizing with HCl, such as Nocardia species and Cryptosporidium.

  9. Cell envelope - Wikipedia

    en.wikipedia.org/wiki/Cell_envelope

    The bacterial cell wall differs from that of all other organisms by the presence of peptidoglycan (poly-N-acetylglucosamine and N-acetylmuramic acid), which is located immediately outside of the cytoplasmic membrane. Peptidoglycan is responsible for the rigidity of the bacterial cell wall and for the determination of cell shape. It is ...