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Unique molecular identifiers (UMIs), or molecular barcodes (MBC) are short sequences or molecular "tags" added to DNA fragments in some next generation sequencing library preparation protocols to identify the input DNA molecule.
A PLANET barcode appears either 12 or 14 digits long. The barcode: identifies mailpiece class and shape; identifies the Confirm Subscriber ID; includes up to 6 digits of additional information that the Confirm subscriber chose, such as a mailing number, mailing campaign ID or customer ID; ends with a check digit
The fundamental aspect of BRB-seq is the optimized sample barcode primers. Each barcoded nucleotide sequence includes an adaptor for primer annealing, a 14-nt long barcode that assigns a unique identifier to each individual RNA sample, and a random 14-nt long UMI that tags each mRNA molecule with a unique sequence to distinguish between original mRNA transcripts and duplicates that result from ...
Tangible items are distinguished from one another by the assignment of a unique identifier in the form of a unique data string and encoded in a bar code placed on the item. An item unique identifier is only assigned to a single item and is never reused. Once assigned to an item, the IUID is never changed even if the item is modified or re ...
Once reverse transcription is complete, the cDNAs from many cells can be mixed together for sequencing; transcripts from a particular cell are identified by each cell's unique barcode. [29] [30] Unique molecular identifier (UMIs) can be attached to mRNA/cDNA target sequences to help identify artifacts during library preparation. [31]
ID or I< are typically used for nationally issued ID cards and IP for passport cards. 3–5: 3: alpha+ < Issuing country or organization (ISO 3166-1 alpha-3 code with modifications) 6–36: 30: alpha+ < Name and surname. If there is more than one name they are separated by single filler. Double filler indicates the end of the primary identifier.
Internal transcribed spacer (ITS) is the spacer DNA situated between the small-subunit ribosomal RNA (rRNA) and large-subunit rRNA genes in the chromosome or the corresponding transcribed region in the polycistronic rRNA precursor transcript.
The barcode scheme does not contain a check digit (in contrast to—for instance—Code 128), but it can be considered self-checking on the grounds that a single erroneously interpreted bar cannot generate another valid character. Possibly the most serious drawback of Code 39 is its low data density: It requires more space to encode data in ...