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Target for MALDI imaging with two conductive-surface microscope slides. Sample preparation is a critical step in imaging spectroscopy. Scientists take thin tissue slices mounted on conductive microscope slides and apply a suitable MALDI matrix to the tissue, either manually or automatically.
Over the past ten years, transformative developments in the laser technology, [4] data acquisition, motor-control software (RastirX [5]), and imaging processing software (MSiReader [6] [7]) have promoted IR-MALDESI as a powerful tool for the direct analysis and MSI of diverse biological, forensic, and pharmaceutical samples.
The term matrix-assisted laser desorption ionization (MALDI) was coined in 1985 by Franz Hillenkamp, Michael Karas and their colleagues. [3] These researchers found that the amino acid alanine could be ionized more easily if it was mixed with the amino acid tryptophan and irradiated with a pulsed 266 nm laser.
Mass spectrometry imaging (MSI) is a technique used in mass spectrometry to visualize the spatial distribution of molecules, as biomarkers, metabolites, peptides or proteins by their molecular masses. After collecting a mass spectrum at one spot, the sample is moved to reach another region, and so on, until the entire sample is scanned.
Koichi Tanaka performed the first successful LDI experiments on proteins. [1] Subsequently, Sunner and Chen used graphite particles of 2-150 μm in size as a substrate and solutions of analytes in glycerol to analyze low molecular weight analytes, peptides, and small proteins by surface-assisted laser desorption/ionization MS (SALDI-MS). [2]
Examples of various lipid species. Lipidomics is the large-scale study of pathways and networks of cellular lipids in biological systems. [1] [2] [3] The word "lipidome" is used to describe the complete lipid profile within a cell, tissue, organism, or ecosystem and is a subset of the "metabolome" which also includes other major classes of biological molecules (such as amino acids, sugars ...
Disrupted sleep and sleep loss interferes with the brain's ability to filter out bad memories, which could contribute to a range of mental health conditions, such as anxiety, depression, and post ...
The use of the SICRIT source for additional in-line post-ionization in AP-MALDI experiments can result in significant signal enhancement in the detection of metabolites in biological sample material or enables detection of small (bio)molecules that are not addressable using MALDI alone. [9] Figure 2: MS Imaging with SICRIT Post-Ionization after ...