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Maxam–Gilbert sequencing is a method of DNA sequencing developed by Allan Maxam and Walter Gilbert in 1976–1977. This method is based on nucleobase-specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides. [1] An example Maxam–Gilbert sequencing reaction.
Methods and Concepts in the Life Sciences/DNA Sequencing; Usage on es.wikipedia.org Secuenciación Maxam-Gilbert; Usage on fa.wikipedia.org توالییابی به روش مکسام-گیلبرت; Usage on gl.wikipedia.org Secuenciación de Maxam e Gilbert; Usage on he.wikipedia.org ריצוף מקסאם-גילברט; Usage on ko.wikipedia.org
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Walter Gilbert and Allan Maxam developed a DNA sequencing method - now called Maxam-Gilbert sequencing - which combined chemicals that cut DNA only at specific bases with radioactive labeling and polyacrylamide gel electrophoresis to determine the sequence of long DNA segments. [3] Allan Maxam and Walter Gilbert’s 1977 paper “A new method ...
The technique known as the “Plus and Minus” method, involved supplying all the components of the DNA but excluding the reaction of one of the four bases needed to complete the DNA. [44] In 1976, Gilbert and Maxam, invented a method for rapidly sequencing DNA while at Harvard, known as the Maxam–Gilbert sequencing. [45]
The first DNA sequencing methods were developed by Gilbert (1973) [8] and Sanger (1975). [9] Gilbert introduced a sequencing method based on chemical modification of DNA followed by cleavage at specific bases whereas Sanger's technique is based on dideoxynucleotide chain termination. The Sanger method became popular due to its increased ...
The DNA template labeled at the 3' or 5' end, depending on the location of the binding site(s). Labels that can be used are: radioactivity and fluorescence. Radioactivity has been traditionally used to label DNA fragments for footprinting analysis, as the method was originally developed from the Maxam-Gilbert chemical sequencing technique.
Allan Maxam and Walter Gilbert's 1977 paper "A new method for sequencing DNA" was honored by a Citation for Chemical Breakthrough Award from the Division of History of Chemistry of the American Chemical Society for 2017. It was presented to the Department of Molecular & Cellular Biology, Harvard University. [36] [24]