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The larger the particles, the faster the elution. The larger molecules simply pass by the pores because those molecules are too large to enter the pores. Larger molecules therefore flow through the column more quickly than smaller molecules, that is, the smaller the molecule, the longer the retention time.
The entry and exit of large molecules from the cell nucleus is tightly controlled by the nuclear pore complexes (NPCs). Although small molecules can enter the nucleus without regulation, [ 1 ] macromolecules such as RNA and proteins require association with transport factors known as nuclear transport receptors , like karyopherins called ...
Exocytosis is the process by which a large amount of molecules are released; thus it is a form of bulk transport. Exocytosis occurs via secretory portals at the cell plasma membrane called porosomes. Porosomes are permanent cup-shaped lipoprotein structures at the cell plasma membrane, where secretory vesicles transiently dock and fuse to ...
Transcytosis is the movement of large molecules across the interior of a cell. This process occurs by engulfing the molecule as it moves across the interior of the cell and then releasing the molecule on the other side. There are two types of transcytosis are receptor-mediated transcytosis (RMT) and adsorptive-mediated transcytosis (AMT).
The nuclear pore complex (NPC), is a large protein complex giving rise to the nuclear pore. Nuclear pores are found in the nuclear envelope that surrounds the cell nucleus in eukaryotic cells. The nuclear envelope is studded by a great number of nuclear pores that give access to various molecules, to and from the nucleoplasm and the
Many methods are used to identify cell lines, including isoenzyme analysis, human lymphocyte antigen (HLA) typing, chromosomal analysis, karyotyping, morphology and STR analysis. [35] One significant cell-line cross contaminant is the immortal HeLa cell line. HeLa contamination was first noted in the early 1960s in non-human culture in the USA.
In a single cycle of the pump, three sodium ions are extruded from and two potassium ions are imported into the cell. Active transport is the movement of a substance across a membrane against its concentration gradient. This is usually to accumulate high concentrations of molecules that a cell needs, such as glucose or amino acids.
Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular intake and uptake of molecules ranging from nanosize particles to small chemical compounds to large fragments of DNA. The "cargo" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions .
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