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MRC-5 (Medical Research Council cell strain 5) is a diploid cell culture line composed of fibroblasts, originally developed from the lung tissue of a 14-week-old aborted white male fetus. [ 1 ] [ 2 ] The cell line was isolated by J.P. Jacobs and colleagues in September 1966 from the seventh population doubling of the original strain, and MRC-5 ...
[11] [13] [14] Descendants of the fibroblast cells from these fetuses have been growing in labs ever since, as the WI-38 and MRC-5 cell lines. They are still used to grow vaccine viruses today. [15] [1] As of March 2017, billions of vaccines have been given that were made using the WI-38 line alone. [16]
Albumin, MRC-5 cells, neomycin sulfate, phenol Rabies vaccine ( RabAvert , Greedo) Amphotericin B , beta-propiolactone , chicken protein , chlortetracycline , human serum albumin , neomycin , ovalbumin , polygeline (processed bovine 14 gelatin ), potassium glutamate
To produce viral vaccines, candidate vaccine viruses are grown in mammalian, avian or insect tissue culture of cells with a finite lifespan. [5] These cells are typically Madin-Darby Canine Kidney cells, [6] but others are also used including monkey cell lines pMK and Vero and human cell lines HEK 293, MRC 5, Per.C6, PMK, and WI-38. [7]
Pages in category "Human cell lines" The following 95 pages are in this category, out of 95 total. ... MRC-5; N. NCI-60 Human Tumor Cell Lines Screen; NCI-H23; NCI ...
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The WI-38 cell line stemmed from earlier work by Hayflick growing human cell cultures. [2]In the early 1960s, Hayflick and his colleague Paul Moorhead at the Wistar Institute in Philadelphia, Pennsylvania discovered that when normal human cells were stored in a freezer, the cells remembered the doubling level at which they were stored and, when reconstituted, began to divide from that level to ...
Many methods are used to identify cell lines, including isoenzyme analysis, human lymphocyte antigen (HLA) typing, chromosomal analysis, karyotyping, morphology and STR analysis. [35] One significant cell-line cross contaminant is the immortal HeLa cell line. HeLa contamination was first noted in the early 1960s in non-human culture in the USA.
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