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Maleimide-mediated methodologies are among the most used in bioconjugation. [5] [6] Due to fast reactions and high selectivity towards cysteine residues in proteins, a large variety of maleimide heterobifunctional reagents are used for the preparation of targeted therapeutics, assemblies for studying proteins in their biological context, protein-based microarrays, or proteins immobilisation. [7]
The MEROPS online database for peptidases and their inhibitors: NEM [permanent dead link ] The bifunctional analogues such as p-NN′-phenylenebismaleimide can be used as cross-linking reagent for cystine residues. see Lutter, L. C., Zeichhardt, H., Kurland, C. G. & Stoffier, G. (1972) Mol. Gen. Genet. 119, 357-366.
Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC) is a heterobifunctional amine-to-sulfhydryl crosslinker, which contains two reactive groups at opposite ends: N-hydroxysuccinimide-ester and maleimide, reactive with amines and thiols respectively.
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18195 Ensembl ENSG00000073969 ENSG00000278174 ENSG00000276262 ENSMUSG00000034187 UniProt P46459 P46460 RefSeq (mRNA) NM_006178 NM_008740 RefSeq (protein) NP_006169 NP_032766 Location (UCSC) Chr 17: 46.59 – 46.76 Mb Chr 11: 103.71 – 103.84 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse N -ethylmaleimide-sensitive factor, also known as NSF or N -ethylmaleimide sensitive fusion ...
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In enzymology, a maleimide hydrolase (EC 3.5.2.16) is an enzyme that catalyzes the chemical reaction maleimide + H 2 O ⇌ {\displaystyle \rightleftharpoons } maleamic acid Thus, the two substrates of this enzyme are maleimide and H 2 O , whereas its product is maleamic acid .
This core structure includes a central maleimide group with two indole groups attached. Examples of bisindolylmaleimide derivatives include: Bisindolylmaleimide I
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