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Buffer capacity falls to 33% of the maximum value at pH = pK a ± 1, to 10% at pH = pK a ± 1.5 and to 1% at pH = pK a ± 2. For this reason the most useful range is approximately p K a ± 1. When choosing a buffer for use at a specific pH, it should have a p K a value as close as possible to that pH.
Good sought to identify buffering compounds which met several criteria likely to be of value in biological research. p K a : Because most biological reactions take place near-neutral pH between 6 and 8, ideal buffers would have p K a values in this region to provide maximum buffering capacity there.
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Borate buffered saline (abbreviated BBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range. Borate buffers have an alkaline buffering capacity in the 8–10 range. Boric acid has a pK a of 9.14 at 25 °C.
McIlvaine buffer is a buffer solution composed of citric acid and disodium hydrogen phosphate, also known as citrate-phosphate buffer. It was introduced in 1921 by the United States agronomist Theodore Clinton McIlvaine (1875–1959) from West Virginia University , and it can be prepared in pH 2.2 to 8 by mixing two stock solutions.
Acid-neutralizing capacity or ANC in short is a measure for the overall buffering capacity against acidification of a solution, e.g. surface water or soil water.. ANC is defined as the difference between cations of strong bases and anions of strong acids (see below), or dynamically as the amount of acid needed to change the pH value from the sample's value to a chosen different value. [1]
A food safety hazard is any biological, chemical, or physical property that may cause a food to be unsafe for human consumption. Identify critical control points A critical control point (CCP) is a point, step, or procedure in a food manufacturing process at which control can be applied and, as a result, a food safety hazard can be prevented ...
MES is used as a buffering agent in biology and biochemistry. It has pK a value of 6.15 at 20 °C. The pH (and pK a at ionic strength I≠0) of the buffer solution changes with concentration and temperature, and this effect may be predicted using online calculators. [2] MES is highly soluble in water. The melting point is approx. 300 °C.