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Colocalization is used in real-time single-molecule fluorescence microscopy to detect interactions between fluorescently labeled molecular species. In this case, one species (e.g. a DNA molecule) is typically immobilized on the imaging surface, and the other species (e.g. a DNA-binding protein) is supplied to the solution.
Colocalization is a visual phenomenon when two molecules of interest are associated with the same structures in the cells and potentially share common functional characteristics. [ 1 ] [ 2 ] [ 3 ] CBS provides researchers with reference tools to verify the results of quantitative colocalization measurements. [ 4 ]
Transcription activator-like (TAL) Effector-Nucleotide Targeter 2.0 (TALE-NT) is a suite of web-based tools that allows for custom design of TAL effector repeat arrays for desired targets and prediction of TAL effector binding sites.
1965 13665 Ensembl ENSG00000134001 ENSMUSG00000021116 UniProt P05198 Q6ZWX6 RefSeq (mRNA) NM_004094 NM_026114 RefSeq (protein) NP_004085 NP_080390 Location (UCSC) Chr 14: 67.36 – 67.39 Mb Chr 12: 78.91 – 78.93 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Eukaryotic translation initiation factor 2 subunit 1 (eIF2α) is a protein that in humans is encoded by the EIF2S1 gene ...
AP-2 complex subunit alpha-1 is a protein that in humans is encoded by the AP2A1 gene. [5] This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP2 adaptors) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small ...
These structures correspond in size and location to the fluorescent bodies confirmed to be scintillons by their light emission, and they show colocalization of anti-luciferase and anti-LBP labeling meaning both bioluminescence proteins are found in the structures. [8]
The image above depicts the conversion from a cosegregation matrix to an adjacency matrix is one use of a matrix in genome architecture mapping where scientists are using cryosectioning to find colocalization between DNA regions, genomes, and/or alleles. In that example, cosegregation is being used to describe the linkage of data to each other ...
The morphometric method is a way to demonstrate cell death in the laboratory. Morphometric measurement provides the result of cell death as a volume, size, weight and length of tissue, organ and the whole organism that compares with before and after the occurrence of cell death. [2]