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Neural stem cell (NSCs) populations can be expanded from frozen stocks and grown in StemPro® NSC SFM complete medium as adherent cultures, or as suspension cultures. In either environment, change the spent culture medium every other day.
Since the discovery of neural stem cells (NSC) in the embryonic and adult mammalian central nervous system (CNS), there have been a growing numbers of tissue culture media and protocols to study and functionally characterize NSCs and its progeny in vitro.
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
MSC-derived neural stem-like cells have been found to exhibit significant neuroprotective effects. 119, 120 In addition, MSCs release paracrine signals that enhance neuronal cell proliferation and the differentiation of human NSCs in vitro in co-culture systems, except for the direct transition of MSCs to neural stem-like cells. 121, 122, 123 ...
Download a digital copy of more than 20 neurobiology validated protocols for neural stem cell culture and differentiation, cell analysis, molecular characterization, and transfection. Review experimental best practices, and troubleshooting advice designed to get you started with your neurobiology research. Download now.
Neurons / metabolism. Neurons / physiology* Phenotype. In this introductory chapter, we provide a general overview of neuronal cell culture. This is a rapidly evolving area of research and we provide an outline and contextual framework for the different chapters of this book.
Neural stem cells (NSC) are self-renewing multipotent stem cells in the nervous system that can differentiate into neurons, oligodendrocytes, and astrocytes. Multipotent neural stem cells reside in the region of the subventricular zone (SVZ) or the hippocampus in the fetal or adult brain.