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Colorimetry is "the science and technology used to quantify and describe physically the human color perception". [1] It is similar to spectrophotometry, but is distinguished by its interest in reducing spectra to the physical correlates of color perception, most often the CIE 1931 XYZ color space tristimulus values and related quantities.
As most definitions of color difference are distances within a color space, the standard means of determining distances is the Euclidean distance.If one presently has an RGB (red, green, blue) tuple and wishes to find the color difference, computationally one of the easiest is to consider R, G, B linear dimensions defining the color space.
Colorimetric assays use reagents that undergo a measurable color change in the presence of the analyte. They are widely used in biochemistry to test for the presence of enzymes, specific compounds, antibodies, hormones and many more analytes. For example, para-Nitrophenylphosphate is converted into a yellow product by alkaline phosphatase enzyme.
The equipment required is a colorimeter, some cuvettes and a suitable color reagent. The process may be automated, e.g. by the use of an AutoAnalyzer or by flow injection analysis . Recently, colorimetric analyses developed for colorimeters have been adapted for use with plate readers to speed up analysis and reduce the waste stream.
A comparison between a typical normalized M cone's spectral sensitivity and the CIE 1931 luminosity function for a standard observer in photopic vision. In the CIE 1931 model, Y is the luminance, Z is quasi-equal to blue (of CIE RGB), and X is a mix of the three CIE RGB curves chosen to be nonnegative (see § Definition of the CIE XYZ color space).
The Munsell color system, showing: a circle of hues at value 5 chroma 6; the neutral values from 0 to 10; and the chromas of purple-blue (5PB) at value 5. In colorimetry, the Munsell color system is a color space that specifies colors based on three properties of color: hue (basic color), value , and chroma (color intensity
Color management is the process of ensuring consistent ... Absolute colorimetry is useful to get an exact specified color (e.g., IBM blue), or to quantify the ...
In astronomy, the color index is a simple numerical expression that determines the color of an object, which in the case of a star gives its temperature. The lower the color index, the more blue (or hotter) the object is. Conversely, the larger the color index, the more red (or cooler) the object is.