Search results
Results from the WOW.Com Content Network
Melting curve analysis is an assessment of the dissociation characteristics of double-stranded DNA during heating. As the temperature is raised, the double strand begins to dissociate leading to a rise in the absorbance intensity, hyperchromicity.
In biochemistry, denaturation is a process in which proteins or nucleic acids lose folded structure present in their native state due to various factors, including application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), agitation, radiation, or heat. [3]
The hyperchromic effect is the striking increase in absorbance of DNA upon denaturation. The two strands of DNA are bound together mainly by the stacking interactions, hydrogen bonds and hydrophobic effect between the complementary bases. The hydrogen bond limits the resonance of the aromatic ring so the absorbance of the sample is limited as well.
Denaturation (biochemistry), a structural change in macromolecules caused by extreme conditions; Denaturation (fissile materials), transforming fissile materials so that they cannot be used in nuclear weapons; Denaturation (food), intentional adulteration of food or drink rendering it unfit for consumption while remaining suitable for other uses
Get answers to your AOL Mail, login, Desktop Gold, AOL app, password and subscription questions. Find the support options to contact customer care by email, chat, or phone number.
The melting process results in an endothermic peak in the DSC curve. The ability to determine transition temperatures and enthalpies makes DSC a valuable tool in producing phase diagrams for various chemical systems. [15] Differential scanning calorimetry can also be used to obtain valuable thermodynamics information about proteins.
Learn more about the AOL app and download it from Google Play. The AOL app is available for Android devices running Android 9.0 or newer. Open the Google Play Store on your device. Type "AOL" in the search field. Choose AOL - News, Mail & Video from the search results. Tap Install. Tap Open. If you're unable to update the AOL app, use the ...
Salman Rushdie on Tuesday came face to face with the man accused of nearly killing him, describing the attack in gruesome detail in court — where he took off his glasses to reveal his blind ...