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This theory has been used as the basis for system-suitability tests, as can be seen in the USP Pharmacopaeia, [42] which are a set of quantitative criteria, which test the suitability of the HPLC system to the required analysis at any step of it.
Nowadays, LC–MS has become one of the most widely used chemical analysis techniques because more than 85% of natural chemical compounds are polar and thermally labile and GC-MS cannot process these samples. [5] As an example, HPLC–MS is regarded as the leading analytical technique for proteomics and pharmaceutical laboratories.
The charged aerosol detector (CAD) is a detector used in conjunction with high-performance liquid chromatography (HPLC) and ultra high-performance liquid chromatography (UHPLC) to measure the amount of chemicals in a sample by creating charged aerosol particles which are detected using an electrometer.
A list of chemical analysis methods with acronyms. A Atomic absorption spectroscopy (AAS) ... (HPLC) High performance liquid chromatography-IR spectroscopy (HPLC-IR) I
Atmospheric pressure chemical ionization chamber cross section. Atmospheric pressure chemical ionization (APCI) is an ionization method used in mass spectrometry which utilizes gas-phase ion-molecule reactions at atmospheric pressure (10 5 Pa), [1] [2] commonly coupled with high-performance liquid chromatography (HPLC). [3]
Fast protein liquid chromatography (FPLC) is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid (the mobile phase) and a porous solid (the stationary phase).
The researcher found that, in this case, reverse phase HPLC, was a better, more sensitive technique despite the time savings in direct injection. [21] Analysis of pharmaceuticals by MLC is also gaining popularity. The selectivity and peak shape of MLC over commonly used ion-pair chromatography is much enhanced. [22]
In practice, the analysis begins with a standard polymerase chain reaction (PCR) in order to amplify the fragment of interest. If the amplified region that exhibits the polymorphism(s) is heterozygous , two kinds of fragments corresponding to the allele and the wild polymorphic allele will be present in the PCR product.
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